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Find below  details of sterilization Indicators  required by a company  in Hong Kong  . Detailed terms and procedure are available at  http://www.nkinfobase.com/sendoffertobuyer.html      Contact us                                                                                                                  

Production specifications:

A) Specification

Item 1 - Supply of Bowie & Dick Test Pack, Single Use

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below, point by point whether their offered item complies fully with the tender specifications. Details must be given should the offered product differ from the specifications.

The Bowie & Dick Test Pack offered shall incorporate the following components, requirements and features. M/D Yes No (Provide details) (Please tick as appropriate)

1. The Bowie & Dick test pack shall be a steam sterilization indicator conforming

to BS EN 867:2001, Part 4: Specification for indicators as an alternative to the

Bowie & Dick test for detection of steam penetration or equivalent standards.

M

2. The pack shall be single/multiple (correspond to clause 4.1.1) use, disposable,

ready to use with no set up time. The pack shall be able to place either way up

in the sterilizer or otherwise indicated. M

3. The pack shall be able to encapsulate air and other non-condensable gases and

has the diagnostic capability to test for successful air removal from high

vacuum porous load sterilizer.

M

4. The test pack shall consist of 2 components

4.1 Standardized Test Load

4.1.1. The test load shall be a pre-assembled pack presented with the

indicator system already incorporated and intended for single

use or intended for multiple use with a new indicator system

to be inserted prior to each test. M

4.1.2. The test load shall contain a process indicator on the outer

wrap to provide immediate identification of the processing.M

4.1.3. The outer wrap of the test load shall be marked with:

4.1.3.1. Sterilization temperature(s) suitable for use

4.1.3.2. Manufacturer’s name

4.1.3.3. Batch number

4.1.3.4. Date of Manufacturing (D)

4.1.3.5. Date of ExpiryM

4.2. Chemical Indicator System

4.2.1 The manufacturer shall provide documentary evidence that the

indicator does not release any substance known to be toxic in

sufficient quantities to cause either a health hazard or a hazard

to the intended properties of the product being sterilized,

before, during or after the sterilization process for which it is

designated. M

4.2.2 There shall be no transfer of ink from indicator system. M

4.2.3 The indicator reagent shall be evenly distributed to cover not

less than 30% of the horizontal surface area of the substrate. M

4.2.4 The pattern of indicator reagent distribution before and after

use shall permit clear interpretation of result. M

4.2.5 The indicator system shall show no discernible colour change

after exposure to dry heat at 140 ± 2°C for not less than 30

min. M

4.2.6 The indicator when tested shall show a uniform colour change

after exposure to dry saturated steam at 134°C for 3.5 min., or

at 121°C for 15 min. or at any other time/temperature

combination and the temperature tolerance shall be +1.5 - 0°C

and the time tolerance shall be ± 5 s.M

  

4.2.7 The endpoint condition of an indicator that has been exposed to

a sterilization process shall not change to an extent that could

lead to an interpretation other than that apparent on removal

from the sterilizer when stored as specified by the manufacturer

for not less than 1 year.M

4.2.8 The indicator shall be marked with the following headings:

4.2.8.1 Date

4.2.8.2 Department

4.2.8.3 Site

4.2.8.4 Machine No.

4.2.8.5 Cycle No.

4.2.8.6 Operator

4.2.8.7 Supervisor

4.2.8.8 Result D

4.2.9 Adjacent to each heading there shall be a clear space not less

than 5 x 20 mm for user to enter the required information at

the time of use.D

5 The manufacturer shall supply information including sufficient instructions

on the use of the indicator to enable correct interpretation of the test results.

M

6 The shelf life of the pack shall not be less than 18 months upon receipt of

goods by the Authority or its representatives

M

7 Manufacturer shall provide evidence of conformity with the BS EN

867:2001, Part 4: Specification for indicators as an alternative to the Bowie

& Dick test for detection of steam penetration standard, or equivalent

standards which shall consist of declaration by the manufacturer and by

independent certification bodies

M

8. Tender sample of 40 nos. shall be submitted with the tender for evaluation. M

 

 

Item 2 - Specification

Supply of Rapid Readout Biological Indicator, for 134șC Gravity Steam Sterilization

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below, point

by point whether their offered item

complies fully with the tender

specifications. Details must be given

should the offered product differ

from the specifications.

The Rapid Readout Biological Indicator is designed for the rapid, reliable

monitoring of steam sterilization processes when used in conjunction with an

Auto-reader

M/D Yes No (Provide details)

(Please tick as appropriate)

1. The biological Indicator shall have a dry spore strip of Bacillus

Stearothermophilus strains ATCC 7953, ATCC 12980, CIP 5281 or NCTC

10003; or other strains or organisms of demonstrated equivalent performance.

M

2. The growth medium shall be contained in a crushable sealed glass ampule. This

medium shall be modified tryptic soy broth with dual indicator system.

M

3. The rapid readout biological indicator shall be designed for the rapid , reliable

monitoring of 1340C gravity cycles.

M

4. One indicator is a non-fluorescent substrate, 4-methylumbelliferyl-alpha-Dglucoside,

and the other is a PH-sensitive indicator dye, bromocresol purple.

M

5. There shall have Macroporous material to concentrate the fluorescence at the

bottom of the vial.

M

6. There shall have a flexible polypropylene vial to hold both the dry spore strip

and the ampule of media.

M

7. There shall have a colored cap with one-way passage for sterilant penetration.

M

8. The air passage shall be covered with a hydrophobic filter as a steam sterilantpermeable

bacterial barrier.

M

9. There shall be a process/chemical indicator on the label of the biological

indicator, which shall change colour when exposed to steam sterilization process.

M

10. Population of Organism shall not less than 1.0 x 105 , Typical Value 1.0 x 106 .

M

11. After sterilization, the vial shall be 'crushed' without spilling to join the growth

media with the processed spore strip and incubated in an incubator with a

reading system.

M

12. A fluorescent reading of the vial with a designed fluorescent reader shall give a

definite 'positive' / 'negative' result. Rectification proof or calibration proof of the

fluorescent reader must be provided to assure the absolute reliability or relative

percentage reliability of the result.

M

13. The final negative processed indicator reading for a fluorescence change shall

not be longer than 3 hours.

M

14. The following information/insert shall appear/available on both the multi-unit

biological indicator packages and the cartons:

14.1 The name and strain of biological indicator organism(s)

14.2 The modes of sterilization to be monitored

14.3 The lot number

14.4 The viable spore population

14.5 The resistance performance characteristics (D-values, survival/kill times)

14.6 Direction for use

14.7 The expiry date

14.8 The recommended storage conditions

14.9 The culture media and condition of use (i.e. temperature and time of

incubation.

14.10 Instruction for disposal

14.11 Name and address of manufacturer and/or distributorM

15. The shelf life of the indicator shall not be less than 18 months upon receipt of

goods by the Authority or its representatives .M

 

 

 

 

16. Tender sample of 150 nos. together with the loan of appropriate incubators (if

required) shall be submitted with the tender for evaluation and upon

confirmation of tender award to users/ hospitals, free of charge. Otherwise, the

offer will not be considered.M

 

 

Item 3 - Specification

Supply of Rapid Readout Biological Indicator, for 134șC Vacuum Assisted Steam Sterilization

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below,

point by point whether their

offered item complies fully with

the tender specifications. Details

must be given should the offered

product differ from the

specifications.

The Rapid Readout Biological Indicator is designed for the rapid, reliable

monitoring of steam sterilization processes when used in conjunction with an

Auto-reader

M/D Yes No (Provide

details)

(Please tick as appropriate)

1. The biological Indicator shall have a dry spore strip of Bacillus

Stearothermophilus strains ATCC 7953, ATCC 12980, CIP 5281 or NCTC

10003; or other strains or organisms of demonstrated equivalent performance.

M

2. The growth medium shall be contained in a crushable sealed glass ampule. This

medium shall be modified tryptic soy broth with dual indicator system.

M

3. The rapid readout biological indicator shall be designed for the rapid , reliable

monitoring of 1340C vaccum assisted cycles.

M

4. One indicator is a non-fluorescent substrate, 4-methylumbelliferyl-alpha-Dglucoside,

and the other is a PH-sensitive indicator dye, bromocresol purple.

M

5. There shall have Macroporous material to concentrate the fluorescence at the

bottom of the vial.

M

6. There shall have a flexible polypropylene vial to hold both the dry spore strip and

the ampule of media.

M

7. There shall have a colored cap with one-way passage for sterilant penetration.

M

8. The air passage shall be covered with a hydrophobic filter as a steam sterilantpermeable

bacterial barrier.

M

9. There shall be a process/chemical indicator on the label of the biological

indicator, which shall change colour when exposed to steam sterilization process.

M

10. Population of Organism shall not less than 1.0 x 105 , Typical Value 1.0 x 106 .

M

11. After sterilization, the vial shall be 'crushed' without spilling to join the growth

media with the processed spore strip and incubated in an incubator with a reading

system.

M

12. A fluorescent reading of the vial with a designed fluorescent reader shall give a

definite 'positive' / 'negative' result. Rectification proof or calibration proof of the

fluorescent reader must be provided to assure the absolute reliability or relative

percentage reliability of the result.

M

13. The final negative processed indicator reading for a fluorescence change shall not

be longer than 3 hours.

M

 

 

Item 3 - Specification

Supply of Rapid Readout Biological Indicator, for 134șC Vacuum Assisted Steam Sterilization

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below,

point by point whether their

offered item complies fully with the

tender specifications. Details must

be given should the offered product

differ from the specifications.

The Rapid Readout Biological Indicator is designed for the rapid, reliable

monitoring of steam sterilization processes when used in conjunction with an

Auto-reader

M/D Yes No (Provide

details)

(Please tick as appropriate)

14. The following information/insert shall appear/available on both the multi-unit

biological indicator packages and the cartons:

14.1 The name and strain of biological indicator organism(s)

14.2 The modes of sterilization to be monitored

14.3 The lot number

14.4 The viable spore population

14.5 The resistance performance characteristics (D-values, survival/kill times)

14.6 Direction for use

14.7 The expiry date

14.8 The recommended storage conditions

14.9 The culture media and condition of use (i.e. temperature and time of

incubation.

14.10 Instruction for disposal

14.11 Name and address of manufacturer and/or distributor M

15. The shelf life of the indicator shall not be less than 18 months upon receipt of

goods by the Authority or its representatives .

M

16. Tender sample of 150 nos. together with the loan of appropriate incubators (if

required) shall be submitted with the tender for evaluation and upon

confirmation of tender award to the users/hospitals free of charge. Otherwise,

the offer will not be considered.

M

 

 

 

Item 4 - Specification

Supply of Rapid Readout Biological Indicator, for 121șC Vacuum Assisted Steam Sterilization

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below,

point by point whether their

offered item complies fully with the

tender specifications. Details must

be given should the offered product

differ from the specifications.

The Rapid Readout Biological Indicator is designed for the rapid, reliable

monitoring of steam sterilization processes when used in conjunction with an

Auto-reader

M/D Yes No (Provide

details)

(Please tick as appropriate)

1. The biological Indicator shall have a dry spore strip of Bacillus

Stearothermophilus strains ATCC 7953, ATCC 12980, CIP 5281 or NCTC

10003; or other strains or organisms of demonstrated equivalent performance.

M

2. The growth medium shall be contained in a crushable sealed glass ampule. This

medium shall be modified tryptic soy broth with dual indicator system.

M

3. The rapid readout biological indicator shall be designed for the rapid , reliable

monitoring of both 1210C vaccum assisted cycles.

M

4. One indicator is a non-fluorescent substrate, 4-methylumbelliferyl-alpha-Dglucoside,

and the other is a PH-sensitive indicator dye, bromocresol purple.

M

5. There shall have Macroporous material to concentrate the fluorescence at the

bottom of the vial.

M

6. There shall have a flexible polypropylene vial to hold both the dry spore strip and

the ampule of media.

M

7. There shall have a colored cap with one-way passage for sterilant penetration.

M

8. The air passage shall be covered with a hydrophobic filter as a steam sterilantpermeable

bacterial barrier.

M

9. There shall be a process/chemical indicator on the label of the biological

indicator, which shall change colour when exposed to steam sterilization process.

M

10. Population of Organism shall not less than 1.0 x 105 , Typical Value 1.0 x 106 .

M

11. After sterilization, the vial shall be 'crushed' without spilling to join the growth

media with the processed spore strip and incubated in an incubator with a reading

system.

M

12. A fluorescent reading of the vial with a designed fluorescent reader shall give a

definite 'positive' / 'negative' result. Rectification proof or calibration proof of the

fluorescent reader must be provided to assure the absolute reliability or relative

percentage reliability of the result.

M

13. The final negative processed indicator reading for a fluorescence change shall not

be longer than 3 hours.

M

14. The following information/insert shall appear/available on both the multi-unit

biological indicator packages and the cartons:

14.1 The name and strain of biological indicator organism(s)

14.2 The modes of sterilization to be monitored

14.3 The lot number

14.4 The viable spore population

14.5 The resistance performance characteristics (D-values, survival/kill times)

14.6 Direction for use

14.7 The expiry date

14.8 The recommended storage conditions

14.9 The culture media and condition of use (i.e. temperature and time of

incubation.

14.10 Instruction for disposal

14.11 Name and address of manufacturer and/or distributor

M

15. The shelf life of the indicator shall not be less than 18 months upon receipt of

goods by the Authority or its representatives .

M

16. Tender sample of 150 nos. together with the loan of appropriate incubators (if

required) shall be submitted with the tender for evaluation and upon

confirmation of tender award to users/ hospitals , free of charge. Otherwise, the

offer will not be considered.

M

 

Item 5 - Specification

Supply of Rapid Readout Biological Indicator, for 121șC Gravity Steam Sterilization

The degree of importance of the clause is denoted by:

Mandatory = M

Desirable = D

Tenderers must indicate below,

point by point whether their

offered item complies fully with

the tender specifications. Details

must be given should the offered

product differ from the

specifications.

The Rapid Readout Biological Indicator is designed for the rapid, reliable

monitoring of steam sterilization processes when used in conjunction with an

Auto-reader

M/D Yes No (Provide

details)

(Please tick as appropriate)

1. The biological Indicator shall have a dry spore strip of Bacillus

Stearothermophilus strains ATCC 7953, ATCC 12980, CIP 5281 or NCTC

10003; or other strains or organisms of demonstrated equivalent performance.

M

2. The growth medium shall be contained in a crushable sealed glass ampule. This

medium shall be modified tryptic soy broth with dual indicator system.

M

3. The rapid readout biological indicator shall be designed for the rapid , reliable

monitoring of 121 0C gravity cycles.

M

4. One indicator is a non-fluorescent substrate, 4-methylumbelliferyl-alpha-Dglucoside,

and the other is a PH-sensitive indicator dye, bromocresol purple.

M

5. There shall have Macroporous material to concentrate the fluorescence at the

bottom of the vial.

M

6. There shall have a flexible polypropylene vial to hold both the dry spore strip and

the ampule of media.

M

7. There shall have a colored cap with one-way passage for sterilant penetration.

M

8. The air passage shall be covered with a hydrophobic filter as a steam sterilantpermeable

bacterial barrier.

M

9. There shall be a process/chemical indicator on the label of the biological

indicator, which shall change colour when exposed to steam sterilization process.

M

10. Population of Organism shall not less than 1.0 x 105 , Typical Value 1.0 x 106 .

M

11. After sterilization, the vial shall be 'crushed' without spilling to join the growth

media with the processed spore strip and incubated in an incubator with a reading

system.

M

12. A fluorescent reading of the vial with a designed fluorescent reader shall give a

definite 'positive' / 'negative' result. Rectification proof or calibration proof of the

fluorescent reader must be provided to assure the absolute reliability or relative

percentage reliability of the result.

M

13. The final negative processed indicator reading for a fluorescence change shall not

be longer than 3 hours.

M

14. The following information/insert shall appear/available on both the multi-unit

biological indicator packages and the cartons:

14.1 The name and strain of biological indicator organism(s)

14.2 The modes of sterilization to be monitored

14.3 The lot number

14.4 The viable spore population

14.5 The resistance performance characteristics (D-values, survival/kill times)

14.6 Direction for use

14.7 The expiry date

14.8 The recommended storage conditions

14.9 The culture media and condition of use (i.e. temperature and time of

incubation.

14.10 Instruction for disposal

14.11 Name and address of manufacturer and/or distributor

M

15. The shelf life of the indicator shall not be less than 18 months upon receipt of

goods by the Authority or its representatives .

M

 

 

16. Tender sample of 150 nos. together with the loan of appropriate incubators (if

required) shall be submitted with the tender for evaluation and upon

confirmation of tender award to users/ hospitals, free of charge. Otherwise, the

offer will not be considered.

M

 

General Specifications Sheet

for

Supply of Sterilization Indicators

Special Notes for Tenderer

Product Offered

Tenderers are requested to submit with tender copies of 2 set (each set in separate file) of the

following for consideration or otherwise offers will not be considered :

1. Certified copy of documentary evidence by an independent laboratory that the product

offered conforms to ISO or equivalent standards applicable to sterilization indicators as

quoted under general specification sheet and individual items.

2. Certified copy of Manufacturer Licence issued by the Government or Health Authority of

the country of origin, if applicable.

3. Certified copy of the authorized certification of compliance to ISO

9001:2000/13485:2003 standards in production and quality assurance system.

4. Quality assurance/control report of the recent lot of the products.

 

B) Estimated Quantity For Three Years

   Item 1: Bowie & Dick Test Pack, Single Use: 81746

   Item 2: Rapid Readout Biological Indicator, for 134 șC,

Gravity Steam Sterilization: 13150

   Item 3: Rapid Readout BiologicalIndicator, for 134C,

Vacuum Assisted Steam Sterilization : 48435

   Item 4: Rapid Readout Biological Indicator, for 121C,

Vacuum Assisted Steam Sterilization: 6000

   Item 5: Rapid Readout Biological Indicator,

 for 121C, Steam Sterilization: 1500

 

C) Sample Quantity :

  Item 1: 41 nos

  Item 2: 151 nos

  Item 3: 151 nos

  Item 4: 151 nos

  Item 5: 151 nos

 

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